Our Services

No matter the project, we provide protein engineering and science support with the dedication and drive of an in-house team member. We work seamlessly with your team to help you achieve your goals.

We’re invested in your success and we’re in it for the long haul.

Protein Engineering

Protein engineering is a broad field of science, encompassing a variety of different techniques from random mutagenesis to rational design and protein large language model (pLLM)-based computational approaches (explore the details). Regardless of approach, the goal is the same: build a better protein by either modifying an existing protein or building a new protein with specific traits or functionality.  Essentially, protein engineering can make working with a protein easier,  enable a protein to be used in a totally new way, or create a de novo protein fit for purpose. 
Tyrala Consulting combines protein engineering expertise with a solid understanding of protein structure-function and practical application.  We match our design approach to the task at hand, taking into account your science, objectives, and downstream application. Our goal is to build you the best protein for the job. Below are examples of past projects to illustrate our capabilities. Please reach out to discuss the specifics of your project and we'll provide a plan tailored to your request.

Proteins we’ve worked on:

  • Antibodies: mAb, Fab, scFv, nanobodies, multispecifics, ADCs, radiotherapeutics
  • Membrane proteins and surface receptors (lipid-anchored, transmembrane, peripheral)
  • Enzymes
  • Cytokines
  • DNA-binding proteins
  • Disordered proteins
  • Chimeras and fusion proteins

Example Engineering Projects:

  • Antibody engineering: reformatting, liability mitigation, conjugation site selection 
  • Antigen and small molecule target protein design
  • Design of multi-domain proteins such as chimeric antigen receptors or multi-specific engagers
  • Alter binding affinity or activity: loss/gain-of-function, enhanced function, or introduction of novel function
  • Increase catalytic turnover for an enzyme
  • Improve solubility and stability
  • Library generation for large scale screens

Let’s discuss your project specifics!

Protein Science

Protein sequence composition is  critical for making a great protein, but it won't get you all the way there. Choice in expression system, purification strategy, and formulation conditions also require careful consideration, and a one-size-fits-all approach rarely applies. Tyrala Consulting provides customizable support to carry you through the full project lifecycle.  We  guide your strategy and manage the experimental workflow at each stage either coordinating work through our network of CROs, guiding and training your internal team, or coming on-site to run experiments in your lab.

Protein Science Services:

  • Strategy and protocol development for protein expression and purification
  • Management of protein production and scale-up
  • Quality assessment, biophysical analysis/analytics
  • Assay development: high-throughput biochemical, enzymatic, protein-protein interactions
  • Protein science infrastructure - we'll help you onboard the necessary tools to bring protein science in-house
  • Advanced Techniques: Membrane protein isolation, refolding, nanodisc assembly; Production of untagged proteins; Extraction of native proteins; Recovery and maintenance of protein complexes

Protein Project Lifecycle:

surface view of a protein structure showing a small molecule binding pocket

Structural Biology

If a picture is worth a thousand words, a three-dimensional protein structure at atomic resolution is priceless. Experimental structural data can make all the difference when you need a precise understanding of complex molecular interactions. This is particularly insightful for the rational design of small molecule therapeutics, and can also provide critical insight for precise modulation of protein-DNA/RNA and protein-protein interactions. 

Tyrala Consulting provides specialized structural biology and structural analysis services aimed at enhancing early drug discovery, enabling design of molecules with improved affinity and specificity and/or identification of differentiating targeting strategies.  We manage experimental structure determination with our trusted CROs to generate protein structures using X-ray crystallography, NMR spectroscopy, or cryo-electron microscopy (cryo-EM), and we provide our clients with a thorough structural analysis, interfacing with our clients' in-house team to answer  key questions and  inform downstream decision-making. 

Scientific Illustration

You’ve made your protein, run your experiments, and gathered compelling data. Now it’s time to communicate your science to senior leadership, investors, collaborators, and other external partners to drive your technology and company forward.
Tyrala Consulting specializes in transforming complex scientific concepts into impactful protein and scientific illustrations for clear, effective communication. We collaborate with clients in biotech and legal sectors to create illustrations that elevate research manuscripts, conference posters, investor presentations, board communications, and intellectual property strategies.

Have a request not detailed here? Please reach out to us.

We’re always up for a good challenge!